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	<title>Comments on: Why doesn&#8217;t honey need to be refrigerated?</title>
	<link>http://bioephemera.com/2007/05/30/why-doesnt-honey-need-to-be-refrigerated/</link>
	<description>the art of biology &#038; the biology of art</description>
	<pubDate>Fri, 12 Mar 2010 03:49:06 +0000</pubDate>
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		<title>By: Arowosola Olaosebikan</title>
		<link>http://bioephemera.com/2007/05/30/why-doesnt-honey-need-to-be-refrigerated/#comment-78991</link>
		<author>Arowosola Olaosebikan</author>
		<pubDate>Thu, 16 Jul 2009 21:36:12 +0000</pubDate>
		<guid>http://bioephemera.com/2007/05/30/why-doesnt-honey-need-to-be-refrigerated/#comment-78991</guid>
		<description>I am happy to know a site like this exists. I am presently doing my final year project in school. I am  working on honey. I will like to know if microbes can grow on products made from honey.
Thanks.</description>
		<content:encoded><![CDATA[<p>I am happy to know a site like this exists. I am presently doing my final year project in school. I am  working on honey. I will like to know if microbes can grow on products made from honey.<br />
Thanks.</p>
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		<title>By: cicada</title>
		<link>http://bioephemera.com/2007/05/30/why-doesnt-honey-need-to-be-refrigerated/#comment-9791</link>
		<author>cicada</author>
		<pubDate>Tue, 05 Jun 2007 09:53:28 +0000</pubDate>
		<guid>http://bioephemera.com/2007/05/30/why-doesnt-honey-need-to-be-refrigerated/#comment-9791</guid>
		<description>Ah, but feeling dumb is part of the pleasure of being alive! At least, that's what I tell myself, since I feel dumb most of the time. Please don't disillusion me.</description>
		<content:encoded><![CDATA[<p>Ah, but feeling dumb is part of the pleasure of being alive! At least, that&#8217;s what I tell myself, since I feel dumb most of the time. Please don&#8217;t disillusion me.</p>
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		<title>By: mdvlist</title>
		<link>http://bioephemera.com/2007/05/30/why-doesnt-honey-need-to-be-refrigerated/#comment-9693</link>
		<author>mdvlist</author>
		<pubDate>Mon, 04 Jun 2007 00:38:57 +0000</pubDate>
		<guid>http://bioephemera.com/2007/05/30/why-doesnt-honey-need-to-be-refrigerated/#comment-9693</guid>
		<description>That's absolutely fascinating.  I never thought of the honey question, either, and I'm very glad you answered it for me before I did so that I could be spared the trouble of wondering and feeling dumb.</description>
		<content:encoded><![CDATA[<p>That&#8217;s absolutely fascinating.  I never thought of the honey question, either, and I&#8217;m very glad you answered it for me before I did so that I could be spared the trouble of wondering and feeling dumb.</p>
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		<title>By: cicada</title>
		<link>http://bioephemera.com/2007/05/30/why-doesnt-honey-need-to-be-refrigerated/#comment-9321</link>
		<author>cicada</author>
		<pubDate>Wed, 30 May 2007 23:46:12 +0000</pubDate>
		<guid>http://bioephemera.com/2007/05/30/why-doesnt-honey-need-to-be-refrigerated/#comment-9321</guid>
		<description>That's interesting!  Who knew Monarchs were so picky?

It's been forever since I did dilution series, so I hope this is in the ballpark: since honey is denser than water (~1.45 kg/L), a 50% w/w dilution of honey in water should yield a final solution that's over 60% water by volume - a pretty big change. I think a much weaker dilution would suffice to get some microorganisms in the honey going. It would definitely be something to keep in mind - you wouldn't want to encourage bacterial growth by diluting a topical honey dressing too much.

My understanding is that the first organisms to exploit diluted honey are wlid yeasts, so if you'd left your Monarch food to ferment, you might have eventually had butterfly wine. . . not that you or the Monarchs would probably want to drink it.

BTW, did you let the butterflies go at the end of your project, or did you have to dissect them? I'll happily kill fruit flies any day, but I think sacrificing butterflies would be depressing.</description>
		<content:encoded><![CDATA[<p>That&#8217;s interesting!  Who knew Monarchs were so picky?</p>
<p>It&#8217;s been forever since I did dilution series, so I hope this is in the ballpark: since honey is denser than water (~1.45 kg/L), a 50% w/w dilution of honey in water should yield a final solution that&#8217;s over 60% water by volume - a pretty big change. I think a much weaker dilution would suffice to get some microorganisms in the honey going. It would definitely be something to keep in mind - you wouldn&#8217;t want to encourage bacterial growth by diluting a topical honey dressing too much.</p>
<p>My understanding is that the first organisms to exploit diluted honey are wlid yeasts, so if you&#8217;d left your Monarch food to ferment, you might have eventually had butterfly wine. . . not that you or the Monarchs would probably want to drink it.</p>
<p>BTW, did you let the butterflies go at the end of your project, or did you have to dissect them? I&#8217;ll happily kill fruit flies any day, but I think sacrificing butterflies would be depressing.</p>
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		<title>By: Steve</title>
		<link>http://bioephemera.com/2007/05/30/why-doesnt-honey-need-to-be-refrigerated/#comment-9320</link>
		<author>Steve</author>
		<pubDate>Wed, 30 May 2007 23:18:19 +0000</pubDate>
		<guid>http://bioephemera.com/2007/05/30/why-doesnt-honey-need-to-be-refrigerated/#comment-9320</guid>
		<description>In grad school, part of my research required that I feed hundreds of captive Monarch butterflies. We diluted honey by about 1/2 , I think it was, and in less than a week, even refrigerated, it would start to spoil.  Interestingly, the butterflies would stop feeding before we could smell the spoilage and we'd mix up a fresh batch of "nectar".</description>
		<content:encoded><![CDATA[<p>In grad school, part of my research required that I feed hundreds of captive Monarch butterflies. We diluted honey by about 1/2 , I think it was, and in less than a week, even refrigerated, it would start to spoil.  Interestingly, the butterflies would stop feeding before we could smell the spoilage and we&#8217;d mix up a fresh batch of &#8220;nectar&#8221;.</p>
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